Določanje klonalnosti limfoidnih proliferacij
Determination of lymphoid proliferation clonality

a new method in comparison to the in house method

Authors

  • Petra Škerl Onkološki inštitut, Ljubljana
  • Ira Koković Onkološki inštitut, Ljubljana
  • Veronika Kloboves-Prevodnik Onkološki inštitut, Ljubljana
  • Srdjan Novaković Onkološki inštitut, Ljubljana

Keywords:

lymphoma diagnostics, lymphoid proliferation clonality

Abstract

Na Oddelku za molekularno diagnostiko smo na področju diagnostike limfomov uvedli dodatno metodo za določanje klonalnosti limfoidnih proliferacij. Tudi nova metoda temelji na verižni reakciji s polimerazo (PCR) in na uporabi različnih konsenzusnih oligonukleotidnih začetnikov. Metoda je validirana in registrirana za diagnostične potrebe (oznaka CE). Z razširjenim naborom oligonukleotidnih začetnikov in uporabo večjega števila začetnikov smo, po naših izračunih, izboljšali občutljivost določanja klonalnosti limfocitov B za 23,1 %, določanje klonalnosti limfocitov T pa za 14,3 %. Teoretično lahko sedaj z obema metodama določimo monoklonalno populacijo limfocitov pri 84,6 % B- in 85,7 % T-limfomov.

Abstract (Eng)

At the Department of Molecular Diagnostics, a new method for determining lymphoid proliferation clonality has been introduced in lymphoma diagnostics. The method is based on PCR and on the use of various consensus primers. It is validated and registered for the use in clinical diagnostics (CE-marked method). Our measurements confirmed that the use of extended number of primers improved the sensitivity of determining the lymphoid clonality of B-lymphocytes and T-lymphocytes by 23.1% and 14.3%, respectively. Both methods may, theoretically, be used in determining monoclonal population of lymphocytes in 84.6% of B- and 85.8 of T-lymphomas.

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Published

2009-12-15

How to Cite

Škerl, P., Koković, I., Kloboves-Prevodnik, V., & Novaković, S. (2009). Determination of lymphoid proliferation clonality: a new method in comparison to the in house method. Onkologija : A Medical-Scientific Journal, 13(2), 133–136. Retrieved from https://revijaonkologija.si/Onkologija/article/view/331

Issue

Vol. 13 No. 2 (2009)

Section

Review Article

License

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